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. 2023 Jan 20;14(1):45. doi: 10.1038/s41419-023-05557-x

Fig. 7. ALDH2 interacted with PGC-1α and modulated nuclear translocation of PGC-1α.

Fig. 7

A The heap map showing the correlations of ALDH2 and the mitochondria and apoptosis-related genes. B Co-immunoprecipitation assay for the interaction of ALDH2 with PGC-1α in ALDH2 overexpression HK-2 cells (n = 3). Input immunoblotted is shown as a control. C Co-immunoprecipitation analysis investigating the interaction of endogenous ALDH2 with PGC-1α in HK-2 cells with or without MA treatment (n = 3). Input immunoblotted is shown as a control. D ALDH2 enhanced nuclear translocation of PGC-1α in WT kidney tissues (WT, n = 3; KO, n = 3). E Immunofluorescent analysis of the location of PGC-1α and ALDH2 in HK-2 cells with or without MA and Alda-1 treatment (n = 3). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001; #P < 0.05. (Con Control, MA maleic acid, M + A maleic acid + Alda-1, A Alda-1, n-PGC-1α nuclear PGC-1α, c-PGC-1α cytoplasmic PGC-1α, WT wild type, KO knockout, NC normal control OE, overexpression).