Figure 1.

Characterization of human liver cells pre- and post-engraftment in the FRG mouse model with single-nucleus RNA sequencing

(A) Schematic representation of the liver lobule. Zone 1 is defined as the region closest to the portal triad, zone 2 spans in between zones 1 and 3, and zone 3 is located near the central vein. (B) Experimental design and UMAP visualization of single human hepatic nuclei. Liver cell types were annotated on the basis of integration with the Aizarani dataset.¹³ (C) UMAP visualization of 7,309 single human hepatic nuclei with predicted cell types. (D) UMAP visualization of the unsupervised, graph-based clustering of the human hepatocyte population before and after engraftment in the FRG model. (E–G) Zonation mapping in the UMAP space of all human single-nucleus hepatocytes (E), the pre-engraftment sample (F), and the post-engraftment hepatocytes (G). (H and I) Log-normalized expression of ASS1 (periportal marker) and CYP2AE1 (pericentral marker) in the UMAP space on human nuclei analyzed before and after engraftment (humanized FRG hepatocytes).