Figure 2.

Immunofluorescence characterization of liver zonation in the FRG model

(A) Representative immunofluorescence analyses of a humanized FRG (hFRG) mouse liver stained with the indicated portal markers (yellow), the pericentral marker glutamine synthetase (GS, cyan), and human GAPDH (red), staining for human hepatocytes. HAL, histidine ammonia-lyase; ASL, argininosuccinate lyase; ASS, argininosuccinate synthetase; CPS1, carbamoyl-phosphate synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Scale bars, 200 μm. (B) Representative immunofluorescence analysis of an hFRG mouse liver stained with human GAPDH (red) and cytokeratin 7 (yellow). Scale bars, 100 μm. (C) Schematic representation of the lentiviral transduction approach followed to genetically track human hepatocyte cluster origin in the hFRG model. (D) Representative immunofluorescence analysis of a hFRG mouse liver repopulated with human primary hepatocytes clonally transduced with lentiviral vectors encoding for Venus, Cerulean, and mCherry. Scale bars, 2,000 μm. (E) Immunofluorescence analysis of the hFRG presented in (D), co-stained with glutamine synthetase (top) and cytokeratin 7 (bottom). Scale bars, 200 μm.