Figure 2.

Analysis of floor plate patterning in hESC-derived neural progenitors at day 16

(A) qPCR analysis of canonical WNT pathway and non-canonical WNT pathway at day 16. ^∗p < 0.05 versus D11; †p < 0.01, †††p < 0.001 versus 1.5 μM CHIR99021 (n = 6 independent experiments).

(B) qPCR analysis of EN1 at day 16. ^∗∗∗p < 0.001 versus D11; ††p < 0.01 versus 1.5 μM CHIR99021 (n = 6 independent experiments).

(C) Immunostaining of LMX1A⁺;EN1⁺ cells. Scale bar, 100 μm.

(D) Quantification of EN1⁺/DAPI⁺ cells. ^∗p < 0.05 versus 1.5 μM CHIR99021 (n = 3 independent experiments).

(E) qPCR analysis of rostral and lateral midbrain markers at day 16. ^∗∗p < 0.01, ^∗∗∗p < 0.001 versus D11; †p < 0.05 versus 1.5 μM CHIR99021 (n = 6 independent experiments).

(F) Violin plots of CORIN, LMX1A, and NGN2 generated from scRNA-seq data of developing human ventral midbrain.

(G) Immunostaining of CORIN⁺;LMX1A⁺ cells and NGN2⁺;LMX1A⁺ cells. Scale bar, 100 μm.

(H and I) Quantification of CORIN⁺/DAPI⁺ cells (H) and NGN2⁺/DAPI⁺ cells (I). ^∗p < 0.05, ^∗∗p < 0.01 versus 1.5 μM CHIR99021 (n = 3 independent experiments).