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. 2022 Dec 15;299(1):102800. doi: 10.1016/j.jbc.2022.102800

Table 2.

Kinetic parameters of the inhibition of HDAC6 by 1

HDAC6 forms	Parameters	1
Human HDAC6	IC₅₀, nM	7.7 ± 0.3
	IC₅₀, nM	8.1 ± 0.3^a
	k_on^app, M⁻¹ min⁻¹ (×10⁵)^b	9.6 ± 0.3
	k_on^app, M⁻¹ min⁻¹ (×10⁵)^b	9.1 ± 0.3^a
	k_off^app, min⁻¹ (×10⁻³)^b	4.6 ± 2.3
	k_off^app, min⁻¹ (×10⁻³)^b	6.7 ± 1.9^a
	k_off^app, min⁻¹ (×10⁻³)^c	3.3 ± 0.2
	K_i^app, nM^d	4.8 ± 2.4
	K_i^app, nM^d	6.9 ± 1.9^a
	Mode of inhibition	Slow-on/slow-off
zHDAC6-CD2 WT	IC₅₀, nM	7.8 ± 0.4
	k_on^app, M⁻¹ min⁻¹ (×10⁵)^b	15.1 ± 0.1
	k_off^app, min⁻¹ (×10⁻³)^b	12.8 ± 1.1
	k_off^app, min⁻¹ (×10⁻³)^c	9.6 ± 1.1
	K_i^app, nM^d	8.5 ± 0.7
	Mode of inhibition	Slow-on/slow-off
zHDAC6-CD2 Y745F	IC₅₀, nM	15.1 ± 0.9
	k_on^app, M⁻¹ min⁻¹ (×10⁵)^b	∼10¹⁰^d
	k_off^app, min⁻¹ (×10⁻³)^c	92.7 ± 3.2
	Mode of inhibition	Fast-on/slow-off

^a

Parameters determined in D₂O.

^b

Calculated from k_obs plot, K_i^app = k_off^app/k_on^app.

^c

k_off^app values from jump-dilution assay.

^d

Parameter estimated from k_off^app/K_i^app ratio.