Table 6.

Effect of Ca and P level (CaP) and phytase supplementation (PHY) on the concentrations of TiO₂ (mg/g), myo-inositol, and inositol phosphates in the ileum (µmol/mg TiO₂) and myo-inositol in blood plasma (µmol/ml) of broilers and turkeys at 21 d of age.

											Myo-inositol
Species	Treatment1		TiO2	InsP6	Ins(1,2,4,5,6)P5	Ins(1,2,3,4,5)P5	Ins(1,2,3,4,6)P5	Ins(1,2,5,6)P4	Ins(1,2,3,4)P4	InsP3x2	Ileum	Blood
Broiler	CaP–	PHY–	15.3b	1.67c	0.03de	0.08	0.03	n.d.3	0.02	n.d.	0.67b	0.27c
		PHY+	15.8b	0.52e	0.03e	0.08	n.d.	0.05	0.01	n.d.	1.60a	0.33b
	CaP+	PHY–	16.1b	2.45a	0.18a	0.12	0.05	0.04	0.03	n.d.	0.14d	0.18e
		PHY+	17.1a	0.89d	0.11c	0.29	< loq4	0.66	0.07	0.37	0.31c	0.26c
Turkey	CaP–	PHY–	14.1c	1.76c	0.05d	0.06	0.03	n.d.	< loq	n.d.	0.11d	0.29c
		PHY+	13.2cd	0.99d	0.04de	0.11	< loq	0.04	0.01	n.d.	0.28c	0.43a
	CaP+	PHY–	12.4d	2.21b	0.15b	0.10	0.05	0.02	0.01	n.d.	0.12d	0.17e
		PHY+	14.0c	1.60c	0.14b	0.29	0.02	0.24	0.04	0.09	0.15d	0.21d
SEM			0.36	0.084	0.007	0.014	0.002	0.024	0.004	0.024	0.028	0.011
P-values
Species			<0.001	<0.001	0.152	1.000	0.092	<0.001	0.004	<0.001	<0.001	0.055
CaP			0.181	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001		<0.001	<0.001
PHY			0.015	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001		<0.001	<0.001
Species × CaP			0.003	0.688	0.152	0.475	0.302	<0.001	0.004		<0.001	<0.001
Species × PHY			0.424	<0.001	0.001	0.095		<0.001	0.702		<0.001	0.260
CaP × PHY			0.003	0.232	0.002	<0.001			<0.001		<0.001	0.014
Species × CaP × PHY			0.030	0.012	<0.001	0.422					<0.001	<0.001

¹Calculated composition: CaP–, 4.1 g P/kg and 5.5 g Ca/kg; CaP+, 9.0 g P/kg and 12.0 g Ca/kg; PHY–, no supplemented phytase; PHY+: 1,500 FTU/kg supplemented phytase.

²Ins(1,2,6)P₃, Ins(1,4,5)P₃, and Ins(2,4,5)P₃ could not be differentiated due to co-elution and are thus referred to as InsP_3x.

³n.d. = not detectable (<0.1 µmol/g DM corresponds to <0.006 µmol/mg TiO₂).

⁴loq = limit of quantification (<0.3 µmol/g DM corresponds to 0.018 µmol/mg TiO₂).

^a-eMeans within a column not sharing a common superscript differ (P < 0.05).