Figure 2.

The tpt-2 mutant showed a delayed HL-response.Fig. 6: Overexpression of KIN10 diminished HL-induction of FB.Fig. 7: Inactivation of SnRK1 induced FB in HL

Kinetic analysis of (A) anthocyanin (represented as cyanidin equivalents), (B) starch (represented as GLC equivalents), (C) GLC, (D) FRC content and gene expression (E–L) in Col-0 (black) and tpt-2 (orange) throughout a 24-h HL shift experiment. Plants were shifted at the end of the night (t0) to the HL condition, and samples were collected at the indicated time points. Gene expression was calculated using the 2^−ΔΔCt method relative to Col-0 (t0) with SAND as the reference gene. Values are means ± standard deviations (n = 4). Statistical significance of differences between WT and tpt-2 at each time point was analyzed using Student’s t test (p < 0.05). Significantly different values are indicated with asterisks inside the tpt-2 symbols. (E–G) Expression levels of representative early biosynthetic genes (EBGs) and (H–J) late biosynthetic genes (LBGs) involved in FB and (K and L) light harvesting chlorophyll A/B binding protein 1.2 (LHCB1.2) and plastocyanin (PC) were analyzed.