Figure 7.

Inactivation of SnRK1 induced FB in HL

(A–D) Phenotype (A) and (B) anthocyanin content (expressed as cyanidin equivalents) after 24 h HL and (C)PAP1 and (D)LDOX expression in snrk1α1-3 amiRNAi KIN11 induced (+) or not (–) induced with β-Est (see methods) after 4 and 8 h HL, respectively. Gene expression was calculated relative to β-Est at EoN using the 2^−ΔΔCt method with SAND as the reference gene. Statistical significance of differences was analyzed using Student’s t test (n ≥ 3) in (B)–(D), and p values are shown.

(E–G) Anthocyanin content after 24 h HL (E) and PAP1(F) and LDOX(G) expression in leaves incubated with 30 mM SOR or 30 mM SUC. For statistical analysis, 2-way ANOVA (Dunnett’s multiple comparisons test, n = 3) was used, and letters indicate significant differences at p < 0.05.

(H and I) Relative expression of PAP1(H) and (I)LDOX in Col-0 after 6 h of HL treatment in the absence (w/o) or presence of 1 μM AZD8055 (AZD). Seedlings were grown on MS plates for 10 d in SD conditions. Gene expression was calculated relative to the control (w/o) using the 2^−ΔΔCt method with SAND as the reference gene. Statistical significance of differences between control and AZD treatment was analyzed using Student’s t test. Values are means ± standard deviations (n = 4), and the p values are shown. For all experiments, plants were shifted to HL at the end of the night.