Arabidopsis rare1 mutants with abolished accD-C794 editing displayed a heat-stress-sensitive phenotype.
(A) A schematic map of the RARE1 locus, illustrating the T-DNA insertion sites within the rare1 mutants.
(B) The editing rate of chloroplast accD transcripts at the C794 site in WT and rare1 mutants.
(C) Seedling phenotypes of wild-type (WT), rare1, and pRARE1:RARE1-c-Myc transgenic plants (complemented) grown at 22°C or 28°C.
(D and E) Chlorophyll content in plants grown at 22°C (D) or 28°C (E).
(F and G) Fv/Fm values of WT, rare1, and complemented lines grown at 22°C (F) or 28°C (G). Error bars indicate SD from three independent repeats. Bars marked with an asterisk (Student’s t-test; ∗p ≤ 0.05, ∗∗p ≤ 0.01) differ significantly from the WT sample.