Figure 3.

Overexpression of the C794-edited accD gene conferred heat tolerance.

(A) Schematic illustration of the accD-fusion construct. Expression of the edited accD gene fused with the chloroplast transit peptide of RPOA and a His-tag is driven by the 35S promoter.

(B) Phenotype of C794-edited accD-overexpressing transgenic plants in the rare1 background.

(C) Chlorophyll contents in transgenic seedlings grown at 22°C or 28°C. Data represent the mean of three replicates ±SD. Asterisks (∗) indicate a statistical difference (∗∗p ≤ 0.01).

(D) Heat-tolerance assay of three edited accD transgenic lines in the WT background.

(E) The number of fertile siliques in the WT and transgenic plants under heat stress.

(F) Effect of heat treatment on editing rate at the accD-C794 site in two independent RARE1-complemented plants with incomplete accD editing.

(G) Effect of heat treatment on RARE1 gene expression.