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. 2022 Dec 5;4(1):100502. doi: 10.1016/j.xplc.2022.100502

Figure 3.

Figure 3

De novo synthesis of membrane proteins in WT, ΔFtsH4, A3, and F4CF strains.

Membranes isolated from radiolabeled cells of WT, ΔFtsH4, A3 (control psbA2 deletion strain), and F4CF were analyzed by 2D CN/SDS–PAGE. The CN gel was scanned in white light (Scan) and for chlorophyll fluorescence (Chl fluor). The 2D gels were stained with Coomassie blue (CB) and developed overnight in a phosphoimager (Autoradiogram). Complexes are designated as in Figure 1. Individual PSI or FtsH complexes are marked by red and blue arrows, respectively. Position of PSI(1) is indicated by a red dashed circle. Samples were loaded on the same OD750nm basis. Sections of CB-stained gels are shown to document equal loading of the samples. ATP s., α/β subunits of ATP synthase.