Figure 5.

Analysis of FLAG-tagged FtsH4 (F4CF) pulldown by 1D and 2D SDS–PAGE.

A total of 20 μl of elution was loaded on both 1D SDS and CN gels, and the CN strip was then separated on the 2D SDS gel. 1D and 2D SDS gels were stained with Coomassie blue (1D, 2D CB) or used for immunodetection (1D, 2D blot). Blots were probed with antibodies specific for FtsH4 and Sll1106 in boxes 1 and 2, respectively. FtsH4 degrad. are putative degradation products of the FtsH4 protease, probably resulting from autoproteolysis. The red dashed rectangle indicates the position of Sll1106. Red dashed lines indicate co-migration of Sll1106 with FtsH4 complexes.