Localization of GFP-tagged FtsH4, Chl, and phycobilins in Synechocystis cells.
(A) Structured illumination microscopy (SIM) of FtsH4–GFP. Axial slices of Synechocystis cells with GFP are in green and Chl in red. The axial slices are 125 nm apart, and the scale bar represents 5 μm.
(B) 3D images obtained using SIM and 3D confocal microscopy (CON). The SIM 3D image was obtained using 2D axial slices 125 nm apart, and 3D confocal microscopy images were obtained using 15 2D slices with a thickness of 150 nm.