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. 2022 Sep 2;4(1):100427. doi: 10.1016/j.xplc.2022.100427

Figure 5.

Figure 5

Effect of AOP2 genes on the GSL biosynthesis pathway.

(A) Neighbor-joining trees of AOP gene families in genomes of 13 species, including the AA genome of CXA, CXB, Z1, ECD04, TCA, Chiifu v3.5, turnip, Chiifu A03, Bras, and PCA, the BB genome of Bni, and the CC genome of Bol, with A. thaliana serving as an outgroup, were constructed by aligning the CDSs with 1000 bootstrap replicates. Three AOP2 genes were present in the turnip (purple circles; Gene0405960, Gene0250680, and Gene0486840) and Chiifu (blue circles; BraA02g028320, BraA09g001360, and BraA03g029140) genomes. Red asterisks and Ψ represent pseudogenes in Bol.

(B) Analysis of the synteny between the AOP2 genes in B. rapa Z1 and turnip. Of the three AOP2 genes in turnip, B. rapa Z1 lacked a collinear gene for Gene0486840.

(C) Pungency-related compound and glucoraphanin content in AOP2-RNAi turnip and Chiifu hairy roots. Error bars indicate the standard deviation (n = 4). Asterisks indicate significant differences between the transgenic turnip/Chiifu hairy roots and the control (Student’s t-test; ∗P < 0.05, ∗∗P < 0.01).