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. 2022 Dec 5;4(1):100501. doi: 10.1016/j.xplc.2022.100501

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Diurnal changes in transcript abundance.

(A) Time points at which samples were collected for RNA-seq in the 16 h light (L):8 h dark (D) growth period (LD) and in continuous light (CL).

(B) Average transcripts per million (TPM) were calculated, expressed relative to the maximum, and hierarchically clustered. A total of 4446 genes were significantly differentially expressed (p < 0.05, |log2(fold change)| > 1) at any time point compared with 23h_D or 23h_CL.

(C) The transcript abundance patterns of known diurnally expressed marker genes are shown.

(D) GO overrepresentation analysis (Fisher’s test, Benjamini–Hochberg p < 0.05) was performed, and the top 15 overrepresented categories are indicated.