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. 2023 Jan 7;9(1):e12820. doi: 10.1016/j.heliyon.2023.e12820

Figure 5.

Figure 5

Beclin–1 suppression by 3–MA reverses radioresistance induced by HIF1A under hypoxia. (A) The survival rate was reduced and radiosensitivity was enhanced when Beclin–1 was suppressed by 3–MA, especially under hypoxic conditions. Colony formation assays were performed in U87 and U251 under normoxia or hypoxia for 16 h. Con: control. (B) The proportion of EdU–stained cells was noticeably decreased when Beclin–1 was suppressed by 3–MA, especially under hypoxic conditions. Upper panel: EdU incorporation was visualized using fluorescence microscopy after 24 h of X–ray irradiation (2Gy/1F). Lower panel: quantification of EdU–positive cells. **P < 0.01, ****P < 0.0001. (C) The number of γ–H2AX foci was significantly increased when Beclin–1 was suppressed by 3–MA, especially under hypoxic conditions. Upper panel: γ–H2AX foci were visualized using confocal laser scanning fluorescence microscopy after 0.5, 4, 8, and 24 h of X–ray irradiation (2Gy/1F). Lower panel: quantification of γ–H2AX foci. * P < 0.05, ****P < 0.0001.