Fig. 3.
Encapsulation in the GroE and thermosome cavities affects folding of the DHFRMp part of the chimera. Representative plots of the observed rate constant, λ, as a function of dihydrofolic acid concentration are shown for the indicated mutants of the DHFRMp part of the chimera inside the GroE cavity. Also shown are such plots for folding of the wild-type version of the DHFRMp part of the chimera inside the cavities of the thermosome from Sulfolobus acidocaldarius formed by α and β subunits ((αβ)4 rings) or only β subunits (β9 rings). The data were fitted to Eq. 4, thereby yielding estimates of the folding and unfolding rate constants. The free energies of folding are provided in Table 1. All the experiments were carried out at least in duplicate. Error bars represent SE.