Fig. 6.

In suit MYL4 overexpression attenuates MYL4 p.E11K caused fibrotic atrial cardiomyopathy. (A, B) Representative images of Masson staining and TUNEL staining of atrial sections. Quantification of fibrotic area and apoptotic ratio were on right panel; n = 5. (C) Apoptotic protein Caspase-3 and BCL-2/BAX were measured by Western blot analysis; n = 4–5. (D) Western blot analysis for autophagy function in atrium; n = 4–5. Ctrl = control; BAX = B-cell lymphoma 2–associated X protein; BCL-2 = B-cell lymphoma 2. Other abbreviations are the same as those in Fig. 1. Data were presented as mean ± SEM. Two-tailed Student's t-test is used to compare two groups; *p < 0.05, **p < 0.001.