Figure 3.

Experimental parameters in regenerating liver tissue of mice

(A) Representative DAPI and GS-stained bright-field micrograph.

(B) A number of similar micrographs were used to study cell size distributions during regeneration after PHx. The blue line represents the model = 512 μm².

(C) Lobule size in control mice (blue) and mice that underwent PHx (red). The area of the lobules increased during regeneration by a factor of approximately two. Accordingly, the volume increased by about a factor of 3 as expected after 2/3 hepatectomy.

(D) Kinetics of proliferation.

(E) Bright-field micrograph with overlaid proliferation quantification averaged for square-shaped regions of 100 × 100 μm (one dot per region) within the lobe micrograph. The color of the dots represents the average fraction of proliferating cells within the corresponding region (green: >30% proliferating cells, red: <10% proliferating cells).

(F and G) Distribution of BrdU-positive cells as a measure for proliferation within (F) the lobe, (G) the individual lobules.