Fig. 2.

Ginsenoside Rd promoted omentin release from adipose tissue and up-regulated the expression of omentin in LPS-induced 3T3-L1 adipocytes. (A) The effect of ginsenoside Rd on omentin content in serum (n = 5). (B) The omentin content in EAT medium from each group was detected by Elisa Kit (n = 5). (C) The effect of the conditioned medium on OGD-induced cardiomyocytes viability was detected by MTT (n = 5). (D) The expression of omentin in 3T3-L1 adipocytes was performed by immunofluorescence staining. Scale bar = 20 μm (n = 5). (E) The protein expression level of omentin was determined by Western blot. β-actin was used as a loading control (n = 5). (F) Omentin luciferase activity with and without Rd treatment. Values are expressed as the means ± SD. One-way ANOVA was followed by the Dunnett's post hoc test. ^###P < 0.001, ^##P < 0.01 vs. Sham or Control; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CAL or LPS.