Activity of PKR is elevated in aging vascular endothelium
(A) PKR mRNA expression determined by real-time qPCR in young and old WT mice (n = 6). Data are represented as mean ± SEM.
(B) Representative immunoblots and densitometric analysis of PKR and p-PKR level in young and old WT mice aortas (n = 5). Data are represented as mean ± SEM.
(C) Representative photomicrographs and statistical analysis of serial sections of arteries from young and old WT mice (n = 5). Sections were immunohistochemical stained for CD31, PKR and p-PKR. Data are represented as mean ± SEM and Scale Bar = 50 μm. ∗∗p< 0.01; ∗∗∗p< 0.001 (unpaired/two-tailed t-test).