Figure 3.

Knockout of PKR inhibits the transforming phenotype of VSMC in aging vessel

(A) Representative immunoblots and densitometric analysis of arterial α-SMA, SM22α, caldesmon, calponin, thrombospondin and osteopontin level in WT and PKR knockout mice of different age (n = 4).

(B) Representative photomicrographs and statistical analysis of serial sections of arteries from WT and PKR knockout mice of different age (n = 5). Sections were immunohistochemical stained for the α-SMA, SM22α and osteopontin (n = 5). Scale Bar = 50 μm. ^∗p< 0.05; ^∗∗p< 0.01; ^∗∗∗p< 0.001 (two-way ANOVA test). Data are represented as mean ± SEM.