TABLE 1.
Bacterial strains and plasmids used in this study
| Straina or plasmid | Relevant propertiesb | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | recA1 gyrA (Nal) Δ(lacIZYA-argF) (Φ80dlacΔlacZM15) | Gibco-BRL |
| HB101 | F−, hsd-20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 (Str) xyl-5 mtl-I supE44 | 9 |
| S. agalactiae | ||
| NEM316 | Serotype III isolated from neonatal blood culture (early onset disease) | 21 |
| NEM1640 | NEM316 sodAΩaphA-3, Km | This work |
| NEM1641 | NEM1640::pAT113/SpΩsodA, Km, Sp | This work |
| NEM1871 | NEM316 cpsDΩaphA-3, Km | This work |
| Plasmids | ||
| pRK24 | Ap, Tc, Tra+, Mob+ (IncP) | 42 |
| pG+host5 | Em; ColE1 replicon, thermosensitive derivative of pGK12, MCS pBluescript | 8 |
| pAT113/Sp | Sp, Mob+ (IncP); oriR pACYC184, attTn1545, lacZα+, MCS pUC19 | 11 |
| pTCV-int | Em, Km, Mob+ (IncP); a pTCV-erm derivative with a 1.8-kb EcoRI-PstI fragment carrying PdltRΩintTn1545 | Submittedc |
a
Bacteria were grown in BHI broth or agar.
b
Ap, Cm, Em, Km, Nal, Sp, Str, and Tc indicate resistance to ampicillin, chloramphenicol, erythromycin, kanamycin, nalidixic acid, spectinomycin, streptomycin, and tetracycline, respectively. attTn and MCS indicate transposon attachment site and multiple-cloning site, respectively.
c
C. Poyart, M.-C. Lamy, C. Boumaila, F. Fiedler, and P. Trieu-Cuot, submitted for publication.