TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′)a | Target | Match positionb |
|---|---|---|---|
| SOD1 | CGGAATTCGGATTATCTCTTTGATTTAGCC | sodA locus | 1729–1750 |
| SOD2 | AAAGGTACCTGTTGCTGCTGCTGTG | sodA locus | 2210–2227 |
| SOD3 | AGAGGATCCAATTATGGAAGGTAAG | sodA locus | 2309–2327 |
| SOD4 | AACCTGCAGTCGTCGTTGAATAGG | sodA locus | 2731–2747 |
| SOD5 | CGGAATTCAAGATGTCTTACTTGGCAGG | sodA locus | 1406–1426 |
| CAPE | AAGAATTCTTACGCTAAGTTTTCACG | cpsD | |
| CAPK | GGGGTACCTATAATAGCACCCGTGAT | cpsD | |
| CAPB | GGGGATCCAGAAAAGATGGTGGACCGGC | cpsD | |
| CAPP | GGCTGCAGATGACCACCACTTGAACC | cpsD | |
| KanK | GGGGTACCTTTAAATACTGTAG | aphA-3 | |
| KanB | TCTGGATCCTAAAACAATTCATCC | aphA-3 | |
| attRin | GGGATATATCAACGGTGG | pAT113/Sp | |
| attRout | GATAAGTCCAGTTTTTATGCGG | pAT113/Sp | |
| attLin | CCTTCTCGTTCGGAGGAAATCC | pAT113/Sp | |
| attLout | TTCTGACAGCTAAGACATGAGG | pAT113/Sp |
a
The restriction sites included in the oligonucleotides for subsequent cloning of the amplified fragments are indicated in bold type.
b
Numbering is according to the nucleotide sequence of the sodA locus (21).