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. 2022 Dec 17;299(1):102812. doi: 10.1016/j.jbc.2022.102812

Figure 5.

Figure 5

CXXC5–CRL4B–NuRD promotes the proliferation of breast cancer cells in vitro and accelerates the growth of breast cancer in vivo.A, EdU assays were performed in MCF-7 cells transfected with the indicated siRNAs and the indicated expression constructs. Figures show representative images and statistical analysis. Each bar represents the mean ± SD from biological triplicate experiments. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. Western blotting was used to detect the indicated protein expression levels. B, growth curve assays of lentivirus-infected MCF-7 cells carrying the knockdown shRNAs and indicated overexpressed constructs. Each bar represents the mean ± SD from biological triplicate experiments. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. C, crystal violet staining was performed after culturing lentivirus-infected MCF-7 cells carrying the indicated overexpressed constructs and knockdown shRNAs for 14 days. Representative images are shown. D, MCF-7 tumors stably expressing shRNA and construction as indicated were transplanted onto ovariectomized athymic mice (n = 5). Representative tumors are shown, and tumor volumes were measured. ∗∗∗p < 0.001. The expression levels of the indicated proteins in these tumors were detected by Western blotting.