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. 2023 Jan 10;12(2):332. doi: 10.3390/plants12020332

Table 1.

Sequence of primers used for the allele-specific assay (PASA). An additional mismatch (A → C, underlined) was introduced to increase primer specificity, but at the third-to-last base of the primers instead of the penultimate base as elsewhere suggested [36].

for/rev Primer Name 5′-3′ Sequence
Reverse UTR3 TGGCTGATGAAAGGCAACAC
Forward AS-Trp ACATTTAGGTATGGTTGTTCACTG
AS-Leu ACATTTAGGTATGGTTGTTCACTT
AS-Met ACATTTAGGTATGGTTGTTCACAT