Table 1.
Sequence of primers used for the allele-specific assay (PASA). An additional mismatch (A → C, underlined) was introduced to increase primer specificity, but at the third-to-last base of the primers instead of the penultimate base as elsewhere suggested [36].
| for/rev | Primer Name | 5′-3′ Sequence |
|---|---|---|
| Reverse | UTR3 | TGGCTGATGAAAGGCAACAC |
| Forward | AS-Trp | ACATTTAGGTATGGTTGTTCACTG |
| AS-Leu | ACATTTAGGTATGGTTGTTCACTT | |
| AS-Met | ACATTTAGGTATGGTTGTTCACAT |