TABLE 3.
Analysis of EHEC strains for A/E lesion formation
| Strain, serotype, source | A/E lesions after 6 ha | Adherence phenotypeb | EspD secretion (MEM)c |
|---|---|---|---|
| ZAP001, O157, human | 5 | LA | ++ |
| ZAP004, O157, human | 3 | DA | ++ |
| ZAP008, O157, human | 3 | DA | ++ |
| ZAP010, O157, human | 4 | LA | +++ |
| ZAP058, O157, human | 9 | LA | +++ |
| ZAP065, O157, human | 2 | DA | +/− |
| ZAP088, O157, human | 5 | LA | ++ |
| ZAP028, O157, bovine | 3 | DA | +/− |
| ZAP032, O157, bovine | 2 | DA | + |
| ZAP034, O157, bovine | 3 | DA | +/− |
| ZAP038, O157, bovine | 2 | DA | +/− |
| ZAP041, O157, bovine | 2 | DA | +/− |
| ZAP052, O157, bovine | 4 | LA | ++ |
| ZAP054, O157, bovine | 1 | DA | +/− |
| ZAP021, O26, bovine | 8 | LA | +++ |
| ZAP078, O26, human | 7 | LA | +++ |
a
Actin rearrangement around the site of attached bacteria was detected by FAS as described in Materials and Methods and previously (26, 31). Results are representative of monolayers and are shown as the number showing actin rearrangement per 10 HeLa cells when viewed at a magnification of ×1,000.
b
Only strains exhibiting LA can be considered as forming microcolonies (29).