Table 1.
Fusion | Avg PhoA 1 | Avg LacZ 1 | PhoA (%) 2 | LacZ (%) 2 | NAR (PhoA:LacZ) 3 | Location 4 |
---|---|---|---|---|---|---|
A85 | 1195.4 | 166.0 | 100 | 20 | 5 | tm |
A180 | 254.4 | 467.3 | 21 | 56 | 0.375 | tm-c |
A263 | 140.5 | 835.4 | 12 | 100 | 0.120 | tm-c |
T201 | 873.5 | 0 | 100 | 0 | >100 | p |
T243 | 11.6 | 684.2 | 0.01 | 100 | <0.01 | c |
1 Alkaline phosphatase (PhoA) and β-galactosidase (LacZ) activities of the fusions were determined as described in Section 3; values represent averages of at least four independent experiments, with two technical repeats each; activity measured for E. coli DH5α carrying the empty vector pPLE01 were subtracted from the presented values. 2 Percentage of PhoA and LacZ activities of the fusions regarding the maximum activity in the set for PssA and PssT. 3 Normalized PhoA:LacZ activity ratio (NAR): >10, periplasmic residue; 0.1–10, transmembrane residue; <0.1, cytoplasmic residue. 4 Proposed locations of the fusion points on the topological map of PssA: tm, transmembrane; p, periplasmic, and c, cytoplasmic.