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. 2023 Jan 5;24(2):1035. doi: 10.3390/ijms24021035

Table 1.

Calculation of normalized PhoA:LacZ activity ratio (NAR) and proposed truncation sites location in a given protein.

Fusion Avg PhoA 1 Avg LacZ 1 PhoA (%) 2 LacZ (%) 2 NAR (PhoA:LacZ) 3 Location 4
A85 1195.4 166.0 100 20 5 tm
A180 254.4 467.3 21 56 0.375 tm-c
A263 140.5 835.4 12 100 0.120 tm-c
T201 873.5 0 100 0 >100 p
T243 11.6 684.2 0.01 100 <0.01 c

1 Alkaline phosphatase (PhoA) and β-galactosidase (LacZ) activities of the fusions were determined as described in Section 3; values represent averages of at least four independent experiments, with two technical repeats each; activity measured for E. coli DH5α carrying the empty vector pPLE01 were subtracted from the presented values. 2 Percentage of PhoA and LacZ activities of the fusions regarding the maximum activity in the set for PssA and PssT. 3 Normalized PhoA:LacZ activity ratio (NAR): >10, periplasmic residue; 0.1–10, transmembrane residue; <0.1, cytoplasmic residue. 4 Proposed locations of the fusion points on the topological map of PssA: tm, transmembrane; p, periplasmic, and c, cytoplasmic.