FIG. 3.

Immunolocalization of TGF-β in sections of liver taken from neutropenic rabbits infected with C. albicans. (A to D) Sections were preblocked with normal goat serum prior to incubation with the rabbit polycolonal CC antibody directed against extracellular TGF-β1 (A and C) and compared with control staining with normal rabbit serum (B and D). (E and F) The murine monoclonal panspecific anti-TGF-β antibody 1D11 was biotinylated and used to detect intracellular, active TGF-β within hepatocytes of C. albicans-infected livers (E) and compared to staining with normal mouse serum (F).