FIG. 5.

Production of active TGF-β by C. albicans-infected human peripheral blood monocytes. In addition to a bioassay of conditioned medium, we performed a SELISA for TGF-β1 as described in Materials and Methods. The results for acid-activated conditioned medium at a 1:2 dilution directly correlate with the level of activity as determined by the inhibition of Mv1lu cell growth. These results suggest that the majority of the TGF-β secreted by Candida-infected monocytes has been processed to a biologically active form. Ratios of yeast to monocytes (T:E) were as indicated and were obtained with the same culture supernatants used for experiments in Fig. 4.