Table 5.

Literature summary of DMR-associated genes at weaning that also show differential methylation in mice at 5 months of age.

Gene	Differential Methylation in Males or Females?	Link to Cardiovascular Development or Disease	Reference
Galnt2	Both	Regulation of HDL cholesterol; promoter hypermethylation associated with coronary heart disease; DNA methylation at GALNT2 associated with sex differences in cardiometabolic diseases	[94,95,96,105]
Glb1	Males	Mutation of GLB1 associated with cardiomyopathy in humans	[106]
Kcnk6	Males	Channel encoded by this gene functions in ventricular repolarization; Gene deficiency in mice leads to pulmonary hypertension	[107,108]
Myo3b	Males	Expressed in cardiac mesoderm stage of cardiac differentiation	[109]
Pebp4	Males	Expressed in adventitial layer of coronary arteries	[110]
Rbfox1	Males	Functions in cardiac gene splicing and is down-regulated in heart failure; copy number variants associated with stress cardiomyopathy; gene variants associated with lower blood pressure	[111,112,113,114]
Adcy5	Females	Adenylyl cyclase 5 exacerbates oxidative stress and cardiomyopathy in response to chronic adrenergic stimulation; knockout of Adcy5 in mice protects against aging-induced cardiomyopathy; enhances myocardial contractility and function during exercise	[115,116,117]
Spock1	Females	Plays a role in calcification of the vasculature; and in collagen deposition during cardiac fibrosis	[118,119]