TABLE 2.
Detection of viable MoPn and chlamydial DNA during primary and reactivated (day 120) infections
| Mice | Treatmenta | Method | No. of positive mice/total no. tested at the following day(s) postinfection:
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3–4 | 7 | 10 | 14 | 21 | 28 | 35 | 42 | 56 | 70 | 84 | 117, 120b | 123, 124 | 126, 127 | 129, 130 | |||
| NOS2−/− | Cy | Culturingc | 15/15 | 5/5 | 5/5 | 5/5 | 4/5 | 2/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/14 | 6/14 | 4/9 | 0/4 |
| PCR-Sb swabd | 5/5 | 4/5 | 4/5 | 1/4 | 4/5 | 4/5 | 5/5 | 5/5 | 2/5 | 5/5 | 5/5 | 13/14 | 1/9 | 1/4 | 0/4 | ||
| PCR-Sb tissuee | 3/3 | 3/4 | 3/3 | ||||||||||||||
| PBS | Culturing | 15/15 | 0/12 | 0/8 | 0/5 | 0/3 | |||||||||||
| PCR-Sb swab | 12/12 | 1/8 | 1/5 | 0/3 | |||||||||||||
| PCR-Sb tissue | 2/3 | 2/2 | 1/3 | ||||||||||||||
| NOS2+/+ | Cy | Culturing | 15/15 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 1/5 | 1/5 | 0/5 | 0/5 | 0/5 | 0/14 | 0/14 | 0/9 | 0/5 |
| PCR-Sb swab | 4/5 | 5/5 | 5/5 | 4/5 | 5/5 | 5/5 | 4/5 | 5/5 | 4/5 | 4/5 | 3/5 | 14/14 | 1/14 | 1/9 | 5/5 | ||
| PCR-Sb tissue | 3/3 | 3/3 | 4/4 | ||||||||||||||
| PBS | Culturing | 15/15 | 0/13 | 0/13 | 0/9 | 0/4 | |||||||||||
| PCR-Sb swab | 5/5 | 12/13 | 0/13 | 2/9 | 4/5 | ||||||||||||
| PCR-Sb tissue | 3/3 | 1/3 | 2/4 | ||||||||||||||
a
Treatments were given at 120 days postinfection.
b
Separate samples were collected on different days from the same animal. Swab samples were collected for PCR-Sb on the first day shown, and swab samples were collected for culturing on the second day.
c
Detection of viable MoPn in cervicovaginal swab samples by culturing in HeLa 229 cells.
d
Detection of chlamydial DNA extracted from cervicovaginal swab samples by PCR followed by Sb hybridization of amplicands.
e
Detection of chlamydial DNA extracted from UGT tissues by PCR followed by Sb hybridization of amplicands.