Figure 6.
Fibrinolytic cross-talk between endothelial microvesicles (EMV) or cells and fibrin-, fibronectin- or platelet-bound plasminogen. Plasmin is formed when fibrin-bound plasminogen (A) or fibronectin-bound plasminogen (B) is cleaved by microvesicles carrying uPA. Plasmin formation is prevented by amiloride (Amil) and a polyclonal antibody directed against uPA (α-uPA) as compared to the IgG isotype control. (C) Platelet-bound plasminogen is activated by a fixed concentration of uPA-bearing microvesicles in a platelet concentration-dependent manner until saturation. The dotted line represents non-specific activity on platelet-bound plasminogen by tPA-bearing cells (mouse cortical neurons) [95]. * Significant changes compared with activation in supernatants. § significant changes compared with activation on at 5 × 105 EMPs (p < 0.05.).