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. 2022 Dec 25;13(1):60. doi: 10.3390/life13010060

Table 1.

Micro-organisms, growth conditions and positive controls used for the agar well-diffusion test.

Microorganisms Growth Conditions Positive Controls
Gram+ bacteria Staphylococcus aureus
WDCM 00034
37 °C—24 h in MHA Tetracycline
30 µg/disc
Bacillus cereus
WDCM 00001
30 °C—24 h in MHA Penicillin G
10 UI/disc
Enterococcus faecalis
WDCM 00087
37 °C—24 h in MHA Tetracycline
30 µg/disc
Listeria monocytogenes
WDCM 00021
37 °C—24 h in MHA Tetracycline
30 µg/disc
Clostridium perfringens
WDCM 00007
37 °C—24–48 h under anaerobic conditions in MHAB Penicillin G
10 UI/disc
Clostridium botulinum
ISS CNRB CL 14NT
37 °C per 24–48 h under anaerobic conditions in MHAB Penicillin G
10 UI/disc
Clostridioides difficile
CDC 20120296
37 °C—24–48 h under anaerobic conditions in MHAB Penicillin G
10 UI/disc
Gram− bacteria Pseudomonas aeruginosa
WDCM 00025
25 °C—24–48 h in MHA Gentamycin
10 µg/disc
Salmonella Enteritidis
WDCM 00097
37 °C—24 h in MHA Tetracycline
30 µg/disc
Escherichia coli
WDCM 00013
37 °C—24 h in MHA Tetracycline
30 µg/disc
Campylobacter jejuni
WDCM 00005
41.5 °C—48 h under microaerobic conditions in MHAB Erythromycin
15 µg/disc