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. 2022 Dec 29;15(1):88. doi: 10.3390/v15010088

Figure 3.

Figure 3

Tha is highly adapted to evade the neuronal innate immune system. (A) Expression of innate immune genes upon Tha and Th2P-4M infection. (B) Expression of innate immune genes upon Tha and Th2P-4M infection with subsequent IFN-α treatment at 24 h post-infection. (A,B) Cells were infected with Tha or Th2P-4M (MOI 5), and gene expression was quantified at 48 h post-infection via qPCR. To obtain hiNeurons, hNSC were differentiated for 21 days, resulting in cultures that contained 85.6% hiNeurons (Table S4). hiMacs were differentiated to hiMicros by co-culturing them for three consecutive weeks in inserts with hiNeurons (see Section 2). All experiments were performed three times (n = 3) independently. Heatmaps present gene expression (ΔΔCT), which was normalized to the expression of the reference gene 18S and the respective mock-infected (A) or IFN-α-treated mock (B). Colour scaling presents differences observed in gene expression (ΔΔCT) between infected and respective non-infected controls.