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Primer information to detect tRF5s using qRT-PCR.
| tRF5–GlyCCC | Sequence | GCAUUGGUGGUUCAGUGGUAGAAUUCUCGCC |
| Forward primer | GCATGGGTGGTTCAGTG | |
| Reverse primer | CGTCGGACTGTAGAACTCTCAAAGC | |
| tRF5–GluCTC | Sequence | UCCCUGGUGGUCUAGUGGUUAGGAUUCGGCGCU |
| Forward primer | TCCCTGGTGGTCTAGTG | |
| Reverse primer | CGTCGGACTGTAGAACTCTCAAAGC | |
| tRF5–CysGCA | Sequence | GGGUAUAGCUCAGUGGUAGAGCAUUUGACUGC |
| Forward primer | AGTGGTAGAGCATTTGACTGC | |
| Reverse primer | CGTCGGACTGTAGAACTCTCAAAGC |
