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Association of B. cepacia with alveolar macrophages in Cftr+/+ and Cftr−/− mice infected with BC7. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed in cold methanol, blocked with 5% normal donkey serum, and treated with anti-B. cepacia antibody as described for Fig. 4. Shown is the association of bacteria with alveolar macrophages harvested from the lungs of Cftr+/+ (a) and Cftr−/− (b) mice.
