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. 2023 Jan 10;24(2):1375. doi: 10.3390/ijms24021375

Figure 6.

Figure 6

Kinetics of the cargo progression and its exit from the GC per se. HeLa cells (A–E) were transfected with VFP and subjected to the E-40-15-40-small (Table 1, 1A). After release of the 15 °C temperature block, cells were incubated at 40 °C for 3′ (B); 6′ (C); 12′ (D); 16 min (E) or were examined immediately after the first 15 °C temperature block (A). Cell were fixed and prepared for immuno-electron microscopy and cryo immunogold labeling with anti-GFP (10 nm gold) antibodies. Human fibroblasts (F–H) were transfected with PFP, subjected to the mini-wave (the E-40-15-40-small) synchronization protocol (Table 1, 1A), and examined using cryosection-based IEM 3 (F), 6 (G), and 12 min (H) after the release of the transport block. Nanogold-labeled specimens (E-nano). (I,J) Graph shows that the exit from the GC per se is linear (red line; see also Table 6). Scale bars: 250 nm (A–D,G,H); 320 nm (E,F).