Table 2.
Cargo Protein | When FRAP Examined, after Release of a Transport Block | Rates of Cargo Diffusion through Golgi (Half-Time; Min; Mean ± SD), According to Amount of Cargo Transported | |
---|---|---|---|
Small | Large | ||
VFP | Early | 6.0 ± 0.9 | 1.4 ± 0.6 |
Late | 5.5 ± 1.1 | 4.9 ± 1.0 | |
PFP | Early | Undetectable | Undetectable |
Late | Undetectable | Undetectable | |
AFP | Early | 0.3 ± 0.05 | ND |
Late | 1.1 ± 0.07 | ND |
ND, not determined. Cells were processed to measure FRAP as described in Methods. Half-time was measured using graphs of FRAP.