Table 7.
Reagents.
| Reagent or Resource | Source | Identifier (Catalog no) |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal αnti-GFP (EM dilution 1:100) | Sigma-Aldrich, Milan, Italy | SAB4301138 |
| Goat polyclonal α-mouse IgG-Cy3 (WB 1:500) | Sigma-Aldrich, Milan, Italy | AP124C |
| Goat polyclonal α-mouse IgG-Cy2 (WB 1:1000) | Abcam, Cambridge, UK | ab97034 |
| Goat polyclonal α-rabbit IgG-Cy2 (WB 1:1000) | Abcam, Cambridge, UK | ab6944 |
| Goat polyclonal α-rabbit IgG-Cy3 (WB 1:1000) | Abcam, Cambridge, UK | ab6939 |
| Antibody secondary Alexa 488, 566 and 633 | Sigma-Aldrich, Milan, Italy | |
| Rabbit polyclonal antibodies against GFP | Abcam, Cambridge, UK | ab6556 |
| Monoclonal antibody against the luminal domain of VSVG | Dr. J. Grunberg. | N/A |
| Mouse monoclonal antibody against the luminal domain of VSVG VSV-G (F-6) | Santa Cruz Biotechnology, Dallas, TX, USA | sc-365019 |
| The I-14 monoclonal antibody against folded VSVG | D.S. Lyles (Wake Forest University School of Medicine) | N/A |
| Rabbit polyclonal antibody against GS15 | MyBioSource, Inc. | MBS2525056 |
| Rabbit polyclonal antibodies against the luminal domain of VSVG | Dr. K. Simons. | N/A |
| Invitrogen rabbit polyclonal antibody against YKT6 | ThermoFisher Scientific | PA5-112789 |
| Mouse Anti-VSV Recombinant Antibody (CBMJV-0164) | Creative Biolabs, Shirley, New York, NY, USA | CBMAB-V208-0167-WJ |
| Rabbit polyclonal antibody against TGN46 | ThermoFisher Scientific, Milan, Italy | PA5-23068 |
| Invitrogen rabbit polyclonal antibody against Transferrin Receptor | ThermoFisher Scientific, Milan, Italy | PA5-83022 |
| Rabbit polyclonal antibody against VAMP3 | ThermoFisher Scientific, Milan, Italy | PA5-116218 |
| Chemicals, peptides, and recombinant proteins | ||
| Epon812 | Sigma-Aldrich, Milan, Italy | 25134-21-8 |
| Cycloheximide | Sigma-Aldrich, Milan, Italy | 01810 |
| GlutaMAX™ Supplement | Thermo Fisher Scientific, Milan, Italy | 35050061 |
| Uranyl acetate | Serva, Catoosa, OK, USA | 6159-44-0 |
| GoldEnhance EM | Nanoprobes, Yaphank, NY, USA | 2114 |
| HQ Silver | Nanoprobes, Yaphank, NY, USA | 2012-45ML |
| Fugene 6 transfection reagent | Roche, Indianapolis, IN, USA. | N/A Purchased in 2009 |
| MatTek chambers | Nalge Nunc International, Naperville, IL, USA | P35G-1.5-14-CGRD |
| CO2 independent medium | Invitrogen, Carlsbad, CA, USA | |
| DPBS with D-glucose and sodium pyruvate | Invitrogen, Waltham, MA, USA | 14287-080. |
| Serum and amino acid-free Earle’s balanced salt solution | Gibco (Now Thermo Fisher Scientific). | 24010043 |
| Dulbecco’s modified Eagle’s medium/F12 | ThermoFisher Scientific, Milan, Italy | 1320033 |
| Fetal bovine serum | Sigma-Aldrich, Milan, Italy | F7524 |
| Pyrrophenone | Santa Cruz Biotechnology Dallas, TX, USA | 341973-06-6 |
| Penicillin | Sigma-Aldrich, Milan, Italy | 1504489 |
| Streptomycin | Sigma-Aldrich, Milan, Italy | 3810-74-0 |
| PEG with the molecular mass of 1500-2000 MW | Sigma-Aldrich, Milan, Italy | 25322-68-3 |
| WGA-HRP | Vector Laboratories Newark, CA, USA | PL-1026-2 |