FIG. 2.

Alignment of MSP2 variant sequences identified in the tick salivary gland-derived A. marginale populations. Multiple amino acid substitutions, insertions, and deletions were detected. Alignment of protein sequences was performed using the program AlignX (Vector NTI Suite V 5.5; InforMax, Inc.) with an engine based on the CLUSTAL W algorithm (27). Conserved amino acids are shown in red, amino acids conserved in 11 to 20 or 21 sequences are shown in blue, and amino acid positions present in 1 to 10 or 21 sequences are shown in black.