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. 2023 Jan 15;12(2):409. doi: 10.3390/plants12020409

Figure 3.

Figure 3

Expression pattern of OsPIN1b gene. GUS staining in each tissue of ProOsPIN1b: GUS transgenic rice. Ten biological replicas were analyzed for each tissue. Scale bars = 1 mm. (A) Maturation region of primary root, (B) elongation zone, meristem zone, and root cap of primary root, (C) stem, (D) leaf, (E) transverse section of leaf sheath, (F) floral organ, (G) the germinated seed for 3 days. (H) qRT-PCR analysis of relative expression level of OsPIN1b in different tissues of WT/NIP. R: root; S: stem; L: leaf; R-S: root-stem junction; FL: flag leaf; YP: Young panicle. OsACTIN was used as an internal control. The data are mean ± SD (n = 3) and asterisks indicate significant differences in different tissues (* p < 0.05, ** p < 0.01; Student’s t-test). (I) qRT-PCR analysis of relative expression level of OsPIN1b in lamina joint at different stages of WT/NIP from one to four weeks. The three independent biological repeats were performed in the qRT-PCR analysis. OsACTIN was used as an internal control. The data are mean ± SD (n = 3) and asterisks indicate the significant differences in different stages (** p < 0.01; Student’s t-test).