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The effect of Chac1 silencing on ferroptosis and cell death of RPE cells treated with hydrogen peroxide. (A) Gene silencing effect of Chac1 siRNA in ARPE-19 cells. (B) GSH assay. (C–F) Representative fluorescent images and quantitative analysis of intracellular iron level in ARPE-19 cells using FerroOrange (orange). (G–L) Cell death analysis using PI staining. Scale bar, 50 μm. n = 5 in each group. * p < 0.05, ** p < 0.01.
