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Identification of cell death by necrosis and apoptosis of a neuroblastoma cell line (SH-SY5Y) exposed for 24 h to oxycodone and naloxone. Viable cells (ANX−/7AAD−) (A,B), apoptotic cells (ANX+/7AAD−) (C,D), necrotic cells (ANX−/7AAD+) (E,F), and late apoptotic and/or necrotic cells (ANX+/7AAD+) (G,H). The results were analyzed by the Kruskal–Wallis test followed by Dunn’s post-test and presented as median ± interquartile interval (n = 3–5). C: control, nonexposed cells.
