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. 2022 Dec 20;11(1):5. doi: 10.3390/vaccines11010005

Figure 4.

Figure 4

B cell repertoire responses. Panel (A): Peripheral blood B cell IGH gene repertoires from peripheral blood mononuclear cell RNA. Three individuals were sampled: a representative healthy control (top row); a patient with representative primary SARS-CoV-2 infection at day 9 and 13 post-onset of symptoms (highlighted by green bar); and reinfected patient at day 14, 18 and 42 (pending) post-onset of symptoms (highlighted by pink bar). Serostatus and days post symptoms onset are given on the right y-axis. Columns indicate the class of each IGH sequence with the IGHV gene indicated on the x-axis. The left y-axis indicates CDR-H3 length in amino acids (AA). Dots indicate B cell clonal lineages. Point color indicates median IGHV somatic hypermutation frequency for each clone, and point size indicates the number of reads in the clone. Points are jittered to decrease over-plotting. Panel (B): The bar plot summarizes single-B cell transcriptome data indicating the antibody isotype expressed by B cells in the reinfected patient’s blood, plotted as the frequency of usage of each isotype.