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. 2023 Jan 16;24(2):1775. doi: 10.3390/ijms24021775

Figure 6.

Figure 6

Decreased locomotor activity in ATP13A2 young flies expressing A53T mutant. Sleep analysis was performed in ATP13A2-silenced young flies (0–3 days after hatching) and old flies (30-day-old) expressing wild-type (WT), A30P and A53T mutant α-syn using Ddc-Gal4 driver. Total length of sleep, fragmentation in sleep indicated by sleep bout number and the average length of sleep indicated by sleep bout length in 12 h light (Day) and 12 h dark (Night) cycle was analyzed in young flies (A,B) and old flies (D,E). The activity index represents fly activity level during the wake periods in day, night and in total for young and old flies (C,F). Total activity in young flies was decreased in A53T flies when ATP13A2 was silenced (C). In old flies, both mutants A30P and A53T showed a decreased night-time sleep with increased sleep fragmentation (E). Bars represent mean values of at least three independent experiments ± the standard error mean (S.E.M.). Each experiment was conducted with 16 flies that were individually recorded using drosophila activity monitor. Differences in means were compared by one-way ANOVA followed by the Newman–Keuls Multiple Comparison post hoc test. *** p < 0.0001, ** p < 0.001, and * p < 0.05.