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. 2023 Jan 13;15(1):219. doi: 10.3390/v15010219

Figure 1.

Figure 1

HEK293 cells were infected with recombinant adenoviruses or an empty adenovirus vector. Proteins produced from adenoviruses were assessed via Western blotting. (a) Schematic representation of recombinant adenoviruses. gD2, 1-306aa, ΔUL25, 310-585aa. (b) Western blot analysis of rAd-gD2ΔUL25 (Lane 1), rAd-gD2 (Lane 2), and empty adenovirus vector (Lane 3) using gD2 rabbit polyclonal antibody. gD2ΔUL25 protein is approximately 67 kDa, and gD2 protein is approximately 36 kDa. (c) Western blot analysis of rAd-ΔUL25 (Lane 1) and empty adenovirus vector (Lane 2) using anti-HA tag antibody. ΔUL25 protein is approximately 32 kDa. M, Precision Plus Protein Dual Color Standards.