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. 2023 Jan 6;15(1):202. doi: 10.3390/pharmaceutics15010202

Figure 2.

Figure 2

Plots showing the vitality and the oxygen consumption rate (OCR) measurements on the astrocytes (46 days of differentiation). Control (Control 2) and PKAN patient (PKAN[Gly420Valfs*30]b) astrocytes were incubated in the presence or absence of MIN-102 for the last three days of differentiation. (A) Cells were incubated with MTT solution, and color absorbance at 570 nm was measured. Data are expressed as % relative to the untreated control astrocytes. One-way ANOVA, *** p < 0.001. Mean ± SD, sixteen replicates for the untreated samples and eight replicates for the MIN-102 treated. (B) Oxygen consumption rate (OCR) was measured with XF96 Extracellular Flux Analyzer (Seahorse). Plot shows OCR normalized on cell numbers. Two-way ANOVA, ** p < 0.01. Mean ± SEM, fourteen replicates for the untreated samples and eight replicates for the MIN-102 treated.