Figure 4.

Oxygen consumption rate (OCR) measurements on astrocytes (50–60 days of differentiation). Controls and PKAN patients’ astrocytes were grown in the presence or absence of 100 nM MIN-102 or 25 μM CoA from day 0 of differentiation. Oxygen consumption rate was measured with XF96 Extracellular Flux Analyzer. (A–C) Plots show OCR normalized on cells number (subject names and conditions are detailed in the figure labels). Unpaired t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Mean ± SEM, at least thirteen replicates per sample.