Figure 3.

Time-course of LC3 puncta formation in C19orf12^{G69Rfs*10/G69Rfs*10} fibroblasts treated with CCCP. Representative images of C19orf12^{G69Rfs*10/G69Rfs*10} (116637) and control NHDF fibroblasts treated for 3, 6, and 24 h with 20 µM CCCP. The green signal indicates LC3-II puncta; the blue signal (DAPI), nuclei (A). Quantification of LC3 puncta fluorescence was performed using ImageJ. n = 10 cells from each of the n = 2 independent experiments were considered for the quantification. p values were calculated with an independent sample t-test. All p values were two-sided with a significance level of 0.05. * p < 0.05, ** p < 0.01 (B).